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  • Determination of hydroxyproline
  • Editor:Fuyang DongCheng Biological Engineer CO. LTD.Date:2018-06-12 15:24 Click:

hydroxyproline test kit can be used to detect serum, culture medium, urine, myocardium, skin, cartilage, liver, etc.

P> 1. Significance of determination: hydroxyproline in collagen accounted for 13.4%, in elastin accounted for a very small amount, no other proteins. The amount of hydroxyproline can reflect the collagen metabolism in connective tissue diseases. Some people used the determination of hydroxyproline in skin as an indicator of screening anti-aging drugs. At the developmental stage, hydroxyproline excretion in urine increases with age, and remains stable after development stops. Hydroxyproline in the elderly is less than that in young people. When rats have spontaneous hypertension and secondary hypertension, the content of hydroxyproline in urine increases. Surgical trauma can lead to increased urinary hydroxyproline excretion, due to high fat, low protein, alcoholism and toxicants, malnutrition, or hepatocyte degeneration and necrosis, through inflammation to increase the number of fibers, hepatic lobules, leading to cirrhosis. When hepatic fibrosis occurs, the main increase in the liver is collagen fibers, and hydroxyproline is unique to collagen fibers. The content of hydroxyproline in the liver can be converted into the content of collagen to reflect the degree of hepatic fibrosis. The decomposition of collagen fibers mainly depends on the action of collagenase and other proteases. Hydroxyproline, the decomposition product of collagen fibers, is excreted from urine, and the content of hydroxyproline in urine is determined, which can reflect the degradation of collagen. By measuring the content of hydroxyproline in tissues and urine, the degree of fibrosis can be judged on the one hand, and the drugs for prevention and treatment can be screened.

2, test method: chemical colorimetry. Simple, easy and fast digestion method

3, test equipment: 721 or 722, 751, 752 spectrophotometer, constant temperature water bath, low speed centrifuge.

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The hydroxyproline test box can be used to measure serum, culture medium, urine, myocardium, skin, ilage, liver, etc.

<1, the significance of determination: hydroxyproline 13.4%, collal, a. Ittle in elastin, other proteins do not exist. Collagen is mostly distributed in skin, tendons, cartilage and blood vessels. Therefore, the amount of hydroxyproline can reflect the collagen metabolism of connective tissue diseases. Indicator of screening anti-aging drugs.During the development stage, the excretion of hydroxyproline in urine increased with age, but it was stable after the development stopped.The hydroxyproline of the elderly is less than that of the young.When rats have spontaneous hypertension and secondar Y hypertension, the content of hydroxyproline in urine increase s. Surgical trauma can lead to an increase in urine hydroxyproline excretion, due to high fat, low protein, alcoholism and toxic substances, malnutrition, or liver cell degeneration and necrosis, through inflammation, fiber increase s, s Egmented liver lobules, leading to liver cirrhosis. In liver fibrosis, the main components in the liver are collagen fibers, and hydroxyproline is specific to collagen fibers. The content of HT is measured, which can be converted in to the content of liver collagen to reflect the degree of liver fibers Rosis.The decomposition of collagen fiber mainly depends on the action of collagenase and other protease, and the decomposition product hydroxyproline is discharged from urine, and the content of hydroxyproline in urine can be measured, which can reflect the degradation of collagen. The content of hydroxyproline in tissues and urine, we can judge the degree of fibrosis on the one hand, and can also screen drugs for prevention and treatment.

2.Test method: chemical colorimetric method.A simple, easy and quick digestion method

3.Instruments for testing:721 or 722, 751, 752 spectrophotometer, constant temperature water bath, low speed centrifuge.